验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-28273 targets mTOR in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | mTOR fusion protein Ag28395 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FRAP1, Mammalian target of rapamycin, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 250-289 kDa |
GenBank Accession Number | NM_004958 |
Gene Symbol | mTOR |
Gene ID (NCBI) | 2475 |
RRID | AB_3672825 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42345 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 mTOR antibody CL488-28273 | Download protocol |
FC protocol for CL Plus 488 mTOR antibody CL488-28273 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |