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验证数据展示

  • IF Staining of HeLa using CL488-28273

    IF Staining of HeLa using CL488-28273

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 mTOR antibody (CL488-28273) at dilution of 1:200.

  • FC experiment of HeLa using CL488-28273

    FC experiment of HeLa using CL488-28273

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human mTOR (CL488-28273) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-28273

    IF Staining of HeLa using CL488-28273

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 mTOR antibody (CL488-28273) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated mTOR Polyclonal antibody

mTOR Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28273

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

FRAP1, Mammalian target of rapamycin, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28273-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28273 targets mTOR in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 mTOR fusion protein Ag28395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名 FRAP1, Mammalian target of rapamycin, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein
计算分子量 289 kDa
观测分子量 250-289 kDa
GenBank蛋白编号NM_004958
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_3672825
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42345
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 mTOR antibody CL488-28273Download protocol
FC protocol for CL Plus 488 mTOR antibody CL488-28273Download protocol
Standard Protocols
Click here to view our Standard Protocols