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nectin 4 Recombinant antibody, PBS Only (Capture)

nectin 4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240009E4

Cat no : 83223-1-PBS

Print datasheet

Synonyms

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, LNIR

验证数据展示

  • Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4, Human Nectin-4/PVRL4 Recombinant Matched Antibody Pair - PBS only. 83223-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0415. 83223-7-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Urine of eight individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 3,920.62 pg/mL with a range of 249.12 - 9,633.39 pg/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. 1 x 10^6 cells/mL were lysated by RIPA buffer, and the supernate was assayed and measured 3.2 ng/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    The mean Nectin-4/PVRL4 concentration was determined to be 102.1 ng/mL in MDA-MB-468 cell extract based on a 3.1 mg/mL extract load and 107.4 ng/mL in MCF-7 cell extract based on a 4.2 mg/mL extract load.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Serum of sixteen individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 750.17 pg/mL with a range of 392.48 - 1,810.23 pg/mL.

  • Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-4-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

  • Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-3-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

产品信息

83223-1-PBS targets nectin 4 as part of a matched antibody pair:

MP00147-1: 83223-1-PBS capture and 83223-4-PBS detection (validated in Cytometric bead array)

MP00147-2: 83223-1-PBS capture and 83223-3-PBS detection (validated in Cytometric bead array)

MP00147-4: 83223-1-PBS capture and 83223-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=8.49 x 10-10M
KOff=2.20 x 10-4M
KOn=2.59 x 105M
Full Name poliovirus receptor-related 4
Synonyms Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, LNIR
Calculated Molecular Weight 510 aa, 55 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).