• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-16815

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

验证数据展示

  • IF Staining of HeLa using CL555-16815

    IF Staining of HeLa using CL555-16815

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 p130Cas / BCAR1 antibody (CL555-16815) at dilution of 1:200.

  • FC experiment of A431 using CL555-16815

    FC experiment of A431 using CL555-16815

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human p130Cas / BCAR1 (CL555-16815) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 16815-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-16815 targets p130Cas / BCAR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen p130Cas / BCAR1 fusion protein Ag10354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2919632
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56945
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Download protocol
FC protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Download protocol
Standard Protocols
Click here to view our Standard Protocols