• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of A431 using CL647-16815

    FC experiment of A431 using CL647-16815

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human p130Cas / BCAR1 (CL647-16815) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-16815

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 16815-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-16815 targets p130Cas / BCAR1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 p130Cas / BCAR1 fusion protein Ag10354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
计算分子量 870 aa, 93 kDa
观测分子量 116 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_2934925
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56945
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 p130Cas / BCAR1 antibody CL647-16815Download protocol
Standard Protocols
Click here to view our Standard Protocols