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验证数据展示

  • WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

    WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    IP experiment of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    IP result of anti-p21 (IP:28248-1-AP, 4ug; Detection:28248-1-AP 1:1000) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using p21 antibody (28248-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

Mouse p21 Polyclonal antibody, PBS Only

p21 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 28248-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28248-1-PBS targets p21 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 p21 fusion protein Ag28394 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1A (P21)
别名 Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号NM_001111099
基因名称 p21
Gene ID (NCBI) 12575
RRIDAB_2881097
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39689
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.