p300 Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230369D12

Cat No : 83078-5-PBS

Synonyms

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)
1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.
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1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.
IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

Size

Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

83078-5-PBS targets p300 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications	IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity	human
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	E1A binding protein p300
Synonyms	EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
Calculated Molecular Weight	264 kDa
GenBank Accession Number	NM_001429
Gene Symbol	EP300
Gene ID (NCBI)	2033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q09472
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C