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p300 Recombinant antibody, PBS Only

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230369D12

Cat no : 83078-5-PBS

Print datasheet

Synonyms

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

验证数据展示

  • IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

产品信息

83078-5-PBS targets p300 in IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name E1A binding protein p300
Synonyms EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
Calculated Molecular Weight 264 kDa
GenBank Accession NumberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C