验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
| Positive FC (Intra) detected in | THP-1 cells |
| Positive ChIP-qPCR detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83078-5-RR targets p300 in WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | E1A binding protein p300 |
| 别名 | EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48 |
| 计算分子量 | 264 kDa |
| 观测分子量 | 300 kDa |
| GenBank蛋白编号 | NM_001429 |
| 基因名称 | EP300 |
| Gene ID (NCBI) | 2033 |
| RRID | AB_3670795 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q09472 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for p300 antibody 83078-5-RR | Download protocol |
| IP protocol for p300 antibody 83078-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
