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验证数据展示

  • IF Staining of HepG2 using CL488-10545

    IF Staining of HepG2 using CL488-10545

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 peroxiredoxin 2 antibody (CL488-10545) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10545

    FC experiment of HeLa using CL488-10545

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated peroxiredoxin 2 Polyclonal antibody (CL488-10545)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10545

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10545 targets peroxiredoxin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 peroxiredoxin 2 fusion protein Ag0835 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxiredoxin 2
别名 PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC003022
基因名称 peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_3672450
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP32119
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 peroxiredoxin 2 antibody CL488-10545Download protocol
FC protocol for CL Plus 488 peroxiredoxin 2 antibody CL488-10545Download protocol
Standard Protocols
Click here to view our Standard Protocols