- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IFN gamma protein
Activity
0.02-0.14 ng/mL EC50
Specific Activity
2x107 IU/mg
Species Reactivity
human,mouse
Purity
>95%
Cat No : HZ-1301
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Validation Data Gallery
Technical Specifications
GeneID | 3458 |
Species | Human |
Expression | HEK293 |
Activity | 0.02-0.14 ng/mL EC50 |
Specific Activity | 2x107 IU/mg |
Purity | >95% |
Endotoxin | <1 EU/μg |
Accession Number | P01579 |
Molecular Mass | 29-32 kDa |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA). |
Background
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Publications
Species | Title |
---|---|
EMBO Mol Med Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis. | |
Virulence The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity. | |
Cancer Sci CPI-203 improves the efficacy of anti-PD-1 therapy by inhibiting the induced PD-L1 overexpression in liver cancer. | |
Clin Immunol Therapeutic polyclonal human CD8+ CD25+ Fox3+ TNFR2+ PD-L1+ regulatory cells induced ex-vivo. | |
Int J Mol Med IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins. | |
PLoS One Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19. |