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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN gamma protein- GMP grade

EC50

0.02-0.14 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg, typically 6.91 x 107 IU/mg

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1301-GMP

Print datasheet

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Validation Data Gallery

  • Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IFN gamma (HZ-1301-GMP) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of GMP recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.02-0.14 ng/mL.

    GMP Recombinant human IFN gamma (HZ-1301-GMP) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of GMP recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.02-0.14 ng/mL.

Technical Specifications

GeneID 3458
Species Human
Expression HEK293
EC50 0.02-0.14 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg, typically 6.91 x 107 IU/mg
Purity > 95%
Endotoxin< 0.1 EU/µg
Accession NumberP01579
Molecular Mass 17 and 21 to 32 kDa reduced and non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 17, 21-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma