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验证数据展示

  • WB analysis using 83854-2-RR (same clone as 83854-2-PBS)

    WB analysis using 83854-2-RR (same clone as 83854-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83854-2-RR (vwf antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

    IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included
  • IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

    IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included
  • IHC staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    IHC staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included
  • IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VWF antibody (83854-2-RR, Clone: 240867D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VWF antibody (83854-2-RR, Clone: 240867D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00810-2

    Cytometric bead array standard curve of MP00810-2

    Cytometric bead array standard curve of MP00810-2, VWF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83854-2-PBS. Detection antibody: 83854-1-PBS. Standard: Ag25578. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83854-2-PBS

    Affinity and Kinetic Characterization of 83854-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83854-2-PBS against Human VWF was performed. The affinity constant is 0.605 nM.

  • Affinity and Kinetic Characterization of 83854-2-RR

    Affinity and Kinetic Characterization of 83854-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83854-2-RR against Human VWF were performed. The affinity constant is 0.965 nM.

VWF Recombinant antibody, PBS Only (Capture)

VWF Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83854-2-PBS

产品说明书

CloneNo. 240867D10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VWF Ready-To-Use IHC Kit. VWF primary antibody included.

产品信息

83854-2-PBS targets VWF as part of a matched antibody pair:

MP00810-2: 83854-2-PBS capture and 83854-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 VWF fusion protein Ag25578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 von Willebrand factor
别名 VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10
观测分子量309-320 kDa
基因名称 VWF
Gene ID (NCBI) 7450
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04275
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).