HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS).

Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H4 antibody (CL488-16047) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Histone H4 (CL488-16047) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

human spleen tissue were subjected to SDS PAGE followed by western blot with 18341-1-AP (HIST1H4F antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

AbFlex Histone H4 antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H4 antibody at 0.01 ug/ml.

AbFlex Histone H4K20me1 antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H4K20me1 antibody at 0.5 ug/ml.

AbFlex Histone H4K20me1 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me1 antibody was applied at 0.1 ug/ml to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

AbFlex Histone H4K20me3 Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from T-47D, mammary epithelial ductal cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H4K20me3 binding sites. The image shows binding across a region of chromosome 19.

AbFlex H4K20me3 antibody (rAb) tested by immunofluorescence. HeLa cell stained with 2ug /mL of AbFlex H4K20me3 antibody (rAb) followed by anti-mouse-IgG-488.

AbFlex Histone H4K20me3 antibody (rAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with AbFlex Histone H4K20me3 antibody (rAb) ) (2 ug/ml dilution).

AbFlex Histone H4K20me3 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H4K20me3 antibody (rAb) antibody for Histone H4K20me3. Various methyl-lysine H4 or H3 peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex Histone H4K20me3antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.