验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, human placenta tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67184-1-Ig targets DNAJA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | DNAJA1 fusion protein Ag2284 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| Synonyms | HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2 |
| Calculated Molecular Weight | 397 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC008182 |
| Gene Symbol | DNAJA1 |
| Gene ID (NCBI) | 3301 |
| RRID | AB_2882480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Euglobulin precipitation |
| UNIPROT ID | P31689 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJA1 antibody 67184-1-Ig | Download protocol |
| IHC protocol for DNAJA1 antibody 67184-1-Ig | Download protocol |
| IF protocol for DNAJA1 antibody 67184-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |