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验证数据展示

  • WB analysis using 67550-1-Ig

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67550-1-Ig

    IHC staining of human breast cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EEF2 antibody included
  • WB analysis using 67550-1-Ig

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67550-1-Ig

    IF Staining of HepG2 using 67550-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67550-1-Ig

产品说明书

CloneNo. 1E4D5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, LoVo cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, 4T1 cells, MCF-7 cells, pig brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 EEF2 fusion protein Ag13859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 2
别名 Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
计算分子量 858 aa, 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC126259
基因名称 EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13639
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.

实验方案

Product Specific Protocols
WB protocol for EEF2 antibody 67550-1-IgDownload protocol
IHC protocol for EEF2 antibody 67550-1-IgDownload protocol
IF protocol for EEF2 antibody 67550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols