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HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 66743-1-Ig

产品说明书

CloneNo. 2D10A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit and More (1)

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (3)

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, rat liver tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, mouse, rat, pig, sheep
免疫原 HO-1/HMOX1 fusion protein Ag21296 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001491
基因名称 HO-1
Gene ID (NCBI) 3162
RRIDAB_2882091
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09601
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

实验方案

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IHC protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IF protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Dev Cell

HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis

Authors - Xiang Peng
WB

Adv Healthc Mater

Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing.

Authors - Qiuyue Ding
mouseWB

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
mouseWB

Clin Immunol

Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia

Authors - Mengting Liu
humanWB,IHC,IP

Oncogene

The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1.

Authors - Ming Gao
mouseWB,IF

EMBO Rep

Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy

Authors - Jie Li
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