MBP tag Monoclonal antibody
MBP tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
4C6H4
验证数据展示
经过测试的应用
Positive WB detected in | Recombinant protein |
Positive IP detected in | Recombinant protein protein |
Positive IF/ICC detected in | Transfected cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66003-1-Ig targets MBP tag in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | recombinant protein |
Cited Reactivity | human |
Immunogen | MBP tag fusion protein Ag0942 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | MBP tag |
Synonyms | Maltose Binding Protein, MBP, MBP Tag |
Calculated Molecular Weight | 40 kDa |
Observed Molecular Weight | 40 kDa |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_11183040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MBP tag antibody 66003-1-Ig | Download protocol |
IP protocol for MBP tag antibody 66003-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Struct Mol Biol CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames | ||
Nat Commun Structural insights into pathogenic mechanism of hypohidrotic ectodermal dysplasia caused by ectodysplasin A variants | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Mol Cell Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination. | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Circ Res Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction. |