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验证数据展示

  • WB analysis of HEK-293 using 66216-1-Ig

    WB analysis of HEK-293 using 66216-1-Ig

    WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells.

  • WB analysis of Jurkat using 66216-1-Ig

    WB analysis of Jurkat using 66216-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 66216-1-Ig

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 66216-1-Ig

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66216-1-Ig

    IHC staining of human tonsillitis using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66216-1-Ig (TLE1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 66216-1-Ig

    IF Staining of SH-SY5Y using 66216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TLE1 antibody (66216-1-Ig, Clone: 2F2H11 ) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66216-1-Ig

Print datasheet

CloneNo. 2F2H11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Transducin-like enhancer protein 1, GRG1, Enhancer of split groucho-like protein 1, 2F2H11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66216-1-Ig targets TLE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TLE1 fusion protein Ag21327 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Synonyms Transducin-like enhancer protein 1, GRG1, Enhancer of split groucho-like protein 1, 2F2H11
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
RRIDAB_2881607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04724
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).

实验方案

Product Specific Protocols
WB protocol for TLE1 antibody 66216-1-IgDownload protocol
IHC protocol for TLE1 antibody 66216-1-IgDownload protocol
IF protocol for TLE1 antibody 66216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols