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  • Featured Product
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CoraLite®555-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H9D1

Cat No : CL555-60330

Print datasheet

Synonyms

FLJ36605, VIM, vimentin

验证数据展示

  • IF Staining of HepG2 using CL555-60330

    IF Staining of HepG2 using CL555-60330

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:400.

  • IF Staining of HeLa using CL555-60330

    IF Staining of HeLa using CL555-60330

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-60330 targets Vimentin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms FLJ36605, VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2919663
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08670
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

实验方案

Product Specific Protocols
IF protocol for CL555 Vimentin antibody CL555-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols