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Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

3H9D1

Cat no : 60330-1-Ig

Print datasheet

Synonyms

VIM, Vimentin



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, ROS1728 cells, Jurkat cells, A549 cells, U2OS cells, U-251 cells
Positive IHC detected inhuman appendicitis tissue, human breast cancer tissue, human colon tissue, human endometrial cancer tissue, human liver cancer tissue, human ovary tumor tissue, human pancreas tissue, human renal cell carcinoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, HepG2 cells, Retinal organoids
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60330-1-Ig targets Vimentin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms VIM, Vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2881439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

实验方案

Product Specific Protocols
WB protocol for Vimentin antibody 60330-1-IgDownload protocol
IHC protocol for Vimentin antibody 60330-1-IgDownload protocol
IF protocol for Vimentin antibody 60330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
humanWB,IP, IF

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
mouseIF

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu
mouseWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
mouseWB

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin