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验证数据展示

  • WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50158-1

    Sandwich ELISA standard curve of MP50158-1

    Sandwich ELISA standard curve of MP50158-1, Human Vimentin Monoclonal Matched Antibody Pair - PBS only. 60330-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0489. 60330-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP50158-1

    Sample test of MP50158-1

    The mean Vimentin concentration was determined to be 3,886.65 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

  • IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50158-1

    Cytometric bead array standard curve of MP50158-1

    Cytometric bead array standard curve of MP50158-1, Vimentin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60330-2-PBS. Detection antibody: 60330-1-PBS. Standard:Ag0489. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO Validated

Vimentin Monoclonal antibody, PBS Only (Detector)

Vimentin Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 60330-1-PBS

Print datasheet

CloneNo. 3H9D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

VIM, 3H9D1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.

产品信息

60330-1-PBS targets Vimentin as part of a matched antibody pair:

MP50158-1: 60330-2-PBS capture and 60330-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms VIM, 3H9D1
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2881439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.