CoraLite®488-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1


human, mouse, rat


FC (Intra), IF and More (1)


CoraLite®488 Fluorescent Dye



Cat No : CL488-60330

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VIM, vimentin


Positive IF detected inHepG2 cells, human tonsillitis tissue
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig


Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery


CL488-60330 targets Vimentin in IHC, IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Cited ApplicationsIF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms VIM, vimentin
Calculated molecular weight 466 aa, 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths491 nm / 516 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.


Product Specific Protocols
IF protocol for CL488 Vimentin antibody CL488-60330Download protocol
FC protocol for CL488 Vimentin antibody CL488-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols


mouseIHC, IF

J Mol Cell Biol

Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice.

Authors - Feng Zheng