CoraLite®488-conjugated Vimentin Monoclonal antibody
Vimentin Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra), IF and More (1)
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
3H9D1
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells, human tonsillitis tissue |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
IHC | See 1 publications below |
IF | See 1 publications below |
产品信息
CL488-60330 targets Vimentin in IHC, IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra), IF |
Cited Applications | IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | Vimentin fusion protein Ag0489 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | vimentin |
Synonyms | VIM, vimentin |
Calculated molecular weight | 466 aa, 54 kDa |
GenBank accession number | BC000163 |
Gene symbol | VIM |
Uniprot | P08670 |
Gene ID (NCBI) | 7431 |
RRID | AB_2883145 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 491 nm / 516 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL488 Vimentin antibody CL488-60330 | Download protocol |
FC protocol for CL488 Vimentin antibody CL488-60330 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Mol Cell Biol Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice. |