CoraLite®488-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra), IF and More (1)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3H9D1

Cat No : CL488-60330

Print datasheet

Synonyms

VIM, vimentin



经过测试的应用

Positive IF detected inHepG2 cells, human tonsillitis tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

CL488-60330 targets Vimentin in IHC, IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Cited ApplicationsIF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Full Name vimentin
Synonyms VIM, vimentin
Calculated molecular weight 466 aa, 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
UniprotP08670
Gene ID (NCBI) 7431
RRIDAB_2883145
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL488 Vimentin antibody CL488-60330Download protocol
FC protocol for CL488 Vimentin antibody CL488-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC, IF

J Mol Cell Biol

Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice.

Authors - Feng Zheng