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Biotin-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

CloneNo.

3H9D1

Cat No : Biotin-60330

Print datasheet

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

验证数据展示

  • IHC staining of human tonsillitis using Biotin-60330

    IHC staining of human tonsillitis using Biotin-60330

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using Biotin-60330

    IHC staining of human tonsillitis using Biotin-60330

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60330 targets Vimentin in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC
Tested Reactivity human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2883066
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08670
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. And this antibody is conjugated with biotin.

实验方案

Product Specific Protocols
IHC protocol for Biotin Vimentin antibody Biotin-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols