验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-60330 targets Vimentin in IHC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Vimentin fusion protein Ag0489 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vimentin |
| 别名 | Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin |
| 计算分子量 | 466 aa, 54 kDa |
| GenBank蛋白编号 | BC000163 |
| 基因名称 | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| RRID | AB_2883066 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08670 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. And this antibody is conjugated with biotin.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin Vimentin antibody Biotin-60330 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
