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WFS1 Polyclonal antibody
WFS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, HEK-293 cells |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11558-1-AP targets WFS1 in WB, IP, IF, IHC, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IF, IHC, ELISA Application Description |
Cited Applications | WB, IF, IHC, coIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | WFS1 fusion protein Ag2114 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Wolfram syndrome 1 (wolframin) |
Synonyms | WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN |
Calculated Molecular Weight | 890 aa, 100 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC030130 |
Gene Symbol | WFS1 |
Gene ID (NCBI) | 7466 |
RRID | AB_2216046 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O76024 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for WFS1 antibody 11558-1-AP | Download protocol |
IHC protocol for WFS1 antibody 11558-1-AP | Download protocol |
IF protocol for WFS1 antibody 11558-1-AP | Download protocol |
IP protocol for WFS1 antibody 11558-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space. | ||