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Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody

CD11a Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/4

Cat no : 65563-1-MR

Print datasheet

Synonyms

Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L

验证数据展示

  • FC experiment of human peripheral blood leukocyte using 65563-1-MR

    FC experiment of human peripheral blood leukocyte using 65563-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11a Mouse IgG2a Recombinant Antibody (65563-1-MR, Clone: TS2/4) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inFC receptor blocked human peripheral blood leukocyte

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65563-1-MR targets CD11a in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen HLA-DR CTL line 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20701
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
FC protocol for CD11a antibody 65563-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols