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TCF12 Monoclonal antibody

TCF12 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B8D4

Cat no : 67523-1-Ig

Print datasheet

Synonyms

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

验证数据展示

  • WB analysis using 67523-1-Ig

    WB analysis using 67523-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 67523-1-Ig

    FC experiment of HepG2 using 67523-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67523-1-Ig targets TCF12 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TCF12 fusion protein Ag6287 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name transcription factor 12
Synonyms TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC050556
Gene Symbol TCF12
Gene ID (NCBI) 6938
RRIDAB_2882743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

实验方案

Product Specific Protocols
WB protocol for TCF12 antibody 67523-1-IgDownload protocol
FC protocol for TCF12 antibody 67523-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols