验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue, HEK-293 cells, pig brain tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67884-1-Ig targets ADC in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, pig, rat |
| 文献引用反应性 | human |
| 免疫原 | ADC fusion protein Ag31049 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | arginine decarboxylase |
| 别名 | ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP |
| 计算分子量 | 460 aa, 50 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC028128 |
| 基因名称 | ADC |
| Gene ID (NCBI) | 113451 |
| RRID | AB_2918641 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96A70 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADC antibody 67884-1-Ig | Download protocol |
| IF protocol for ADC antibody 67884-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
