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验证数据展示

  • IF Staining of A431 using CL488-67884

    IF Staining of A431 using CL488-67884

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 ADC antibody (CL488-67884, Clone: 1A2B11 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-67884

    FC experiment of A431 using CL488-67884

    1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ADC (CL488-67884, Clone:1A2B11) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated ADC Monoclonal antibody

ADC Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67884

产品说明书

CloneNo. 1A2B11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

AZIN2, AzI2, Arginine decarboxylase, ARGDC, Antizyme inhibitor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67884-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67884 targets ADC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ADC fusion protein Ag31049 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 arginine decarboxylase
别名 AZIN2, AzI2, Arginine decarboxylase, ARGDC, Antizyme inhibitor 2
计算分子量 460 aa, 50 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC028128
基因名称 ADC
Gene ID (NCBI) 113451
RRIDAB_2923815
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96A70
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ADC antibody CL488-67884Download protocol
FC protocol for CL Plus 488 ADC antibody CL488-67884Download protocol
Standard Protocols
Click here to view our Standard Protocols