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ADC Monoclonal antibody

ADC Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B11

Cat no : 67884-1-Ig

Print datasheet

Synonyms

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

验证数据展示

  • WB analysis of HEK-293T using 67884-1-Ig

    WB analysis of HEK-293T using 67884-1-Ig

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67884-1-Ig

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67884-1-Ig

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67884-1-Ig

    IF Staining of A431 using 67884-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue, HEK-293 cells, pig brain tissue
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67884-1-Ig targets ADC in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, pig, rat
Immunogen ADC fusion protein Ag31049 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name arginine decarboxylase
Synonyms ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP
Calculated Molecular Weight 460 aa, 50 kDa
Observed Molecular Weight48-50 kDa
GenBank Accession NumberBC028128
Gene Symbol ADC
Gene ID (NCBI) 113451
RRIDAB_2918641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96A70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.

实验方案

Product Specific Protocols
WB protocol for ADC antibody 67884-1-IgDownload protocol
IF protocol for ADC antibody 67884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols