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验证数据展示

  • WB analysis using 68069-1-Ig

    WB analysis using 68069-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68069-1-Ig

    IF Staining of HeLa using 68069-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68069-1-Ig

    IF Staining of HeLa using 68069-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

ARF1 Monoclonal antibody

ARF1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68069-1-Ig

产品说明书

CloneNo. 1C10E3
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA and More (1)

别名

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARF1 Ready-To-Use IHC Kit. ARF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68069-1-Ig targets ARF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原 ARF1 fusion protein Ag28021 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor 1
别名 EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3
计算分子量 21 kDa
观测分子量18-21 kDa
GenBank蛋白编号BC009247
基因名称 ARF1
Gene ID (NCBI) 375
RRIDAB_2918810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP84077
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).

实验方案

Product Specific Protocols
WB protocol for ARF1 antibody 68069-1-IgDownload protocol
IF protocol for ARF1 antibody 68069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Metab

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation

Authors - Jian Zhou
View Article