验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68069 targets ARF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, rabbit |
| 免疫原 | ARF1 fusion protein Ag28021 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor 1 |
| 别名 | EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 18-21 kDa |
| GenBank蛋白编号 | BC009247 |
| 基因名称 | ARF1 |
| Gene ID (NCBI) | 375 |
| RRID | AB_2919562 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P84077 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARF1 antibody CL488-68069 | Download protocol |
| FC protocol for CL Plus 488 ARF1 antibody CL488-68069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |