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验证数据展示

  • FC experiment of HeLa using CL488-68069

    FC experiment of HeLa using CL488-68069

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ARF1 (CL488-68069, Clone:1C10E3) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-68069

    IF Staining of HeLa using CL488-68069

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ARF1 antibody (CL488-68069, Clone: 1C10E3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ARF1 Monoclonal antibody

ARF1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68069

Print datasheet

CloneNo. 1C10E3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

IF/ICC, FC (Intra)

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARF1 Ready-To-Use IHC Kit. ARF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68069-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68069 targets ARF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen ARF1 fusion protein Ag28021 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor 1
Synonyms EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC009247
Gene Symbol ARF1
Gene ID (NCBI) 375
RRIDAB_2919562
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP84077
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARF1 antibody CL488-68069Download protocol
FC protocol for CL Plus 488 ARF1 antibody CL488-68069Download protocol
Standard Protocols
Click here to view our Standard Protocols