CoraLite®594-conjugated ARF1 Monoclonal antibody
ARF1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, rabbit
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1C10E3
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ARF1 Ready-To-Use IHC Kit. ARF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68069-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-68069 targets ARF1 in FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, rabbit |
| Immunogen | ARF1 fusion protein Ag28021 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ADP-ribosylation factor 1 |
| Synonyms | EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | BC009247 |
| Gene Symbol | ARF1 |
| Gene ID (NCBI) | 375 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P84077 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 ARF1 antibody CL594-68069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |