ARF1 Monoclonal antibody
ARF1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1C10E3
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IP | See 1 publications below |
产品信息
68069-1-Ig targets ARF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human |
Immunogen | ARF1 fusion protein Ag28021 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ADP-ribosylation factor 1 |
Synonyms | EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 18-21 kDa |
GenBank Accession Number | BC009247 |
Gene Symbol | ARF1 |
Gene ID (NCBI) | 375 |
RRID | AB_2918810 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P84077 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ARF1 antibody 68069-1-Ig | Download protocol |
IF protocol for ARF1 antibody 68069-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation |