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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68069-1-Ig (same clone as 68069-1-PBS)

    WB analysis using 68069-1-Ig (same clone as 68069-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

ARF1 Monoclonal antibody, PBS Only

ARF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68069-1-PBS

产品说明书

CloneNo. 1C10E3

宿主/亚型

Mouse / IgG1

种属反应性

rabbit, pig, rat, mouse, human

应用

WB, IF/ICC, Indirect ELISA

别名

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARF1 Ready-To-Use IHC Kit. ARF1 primary antibody included.

产品信息

68069-1-PBS targets ARF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 ARF1 fusion protein Ag28021 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor 1
别名 EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3
计算分子量 21 kDa
观测分子量18-21 kDa
GenBank蛋白编号BC009247
基因名称 ARF1
Gene ID (NCBI) 375
RRIDAB_2918810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP84077
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).