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SAT1 Recombinant antibody

SAT1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240157G2

Cat No : 83319-1-RR

Print datasheet

Synonyms

DC21, Diamine acetyltransferase 1, KFSD, Putrescine acetyltransferase, SAT, SAT1, SSAT, SSAT 1

验证数据展示

  • WB analysis using 83319-1-RR

    WB analysis using 83319-1-RR

    Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 83319-1-RR

    WB analysis of K-562 using 83319-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 83319-1-RR

    IF Staining of RAW 264.7 using 83319-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells
Positive IF detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83319-1-RR is SAT1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen SAT1 fusion protein Ag1154 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name spermidine/spermine N1-acetyltransferase 1
Synonyms DC21, Diamine acetyltransferase 1, KFSD, Putrescine acetyltransferase, SAT, SAT1, SSAT, SSAT 1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 15-25 kDa
GenBank Accession NumberBC008424
Gene Symbol SAT1
Gene ID (NCBI) 6303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21673
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

实验方案

Product Specific Protocols
WB protocol for SAT1 antibody 83319-1-RRDownload protocol
IF protocol for SAT1 antibody 83319-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols