• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84248-4-RR (same clone as 84248-4-PBS)

    WB analysis using 84248-4-RR (same clone as 84248-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84248-4-RR (same clone as 84248-4-PBS)

    FC experiment of A431 using 84248-4-RR (same clone as 84248-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01144-1

    Cytometric bead array standard curve of MP01144-1

    Cytometric bead array standard curve of MP01144-1, MAGEA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84248-4-PBS. Detection antibody: 84248-5-PBS. Standard: Ag3193. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84248-4-RR

    Affinity and Kinetic Characterization of 84248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

MAGEA4 Recombinant antibody, PBS Only (Capture)

MAGEA4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84248-4-PBS

产品说明书

CloneNo. 241558G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84248-4-PBS targets MAGEA4 as part of a matched antibody pair:

MP01144-1: 84248-4-PBS capture and 84248-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MAGEA4 fusion protein Ag3193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4
计算分子量 317 aa, 35 kDa
观测分子量45 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43358
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.