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验证数据展示

  • FC experiment of HCT 116 using 84292-4-RR (same clone as 84292-4-PBS)

    FC experiment of HCT 116 using 84292-4-RR (same clone as 84292-4-PBS)

    1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

  • WB analysis using 84292-4-RR (same clone as 84292-4-PBS)

    WB analysis using 84292-4-RR (same clone as 84292-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01169-3

    Sandwich ELISA standard curve of MP01169-3

    Sandwich ELISA standard curve of MP01169-3, Human ADAM17 Recombinant Matched Antibody Pair - PBS only. 84292-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32418. 84292-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP01169-3

    Sample test of MP01169-3

    The mean ADAM17 concentration was determined to be 18.51 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load and 13.76 ng/mL in A549 cell extract based on a 2.7 mg/mL extract load.

  • Sample test of MP01169-3

    Sample test of MP01169-3

    Serum of six individual healthy human donors was measured. The human ADAM17 concentration of detected samples was determined to be 1,898.9 pg/mL with a range of 21.5 - 8,096.2 ng/mL.

  • Affinity and Kinetic Characterization of 84292-4-RR

    Affinity and Kinetic Characterization of 84292-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

ADAM17 Recombinant antibody, PBS Only (Detector)

ADAM17 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84292-4-PBS

产品说明书

CloneNo. 241305A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ADAM17 Ready-To-Use IHC Kit. ADAM17 primary antibody included.

产品信息

84292-4-PBS targets ADAM17 as part of a matched antibody pair:

MP01169-3: 84292-3-PBS capture and 84292-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 ADAM17 fusion protein Ag32418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADAM metallopeptidase domain 17
别名 241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86
计算分子量 824 aa, 93 kDa
GenBank蛋白编号BC136783
基因名称 ADAM17
Gene ID (NCBI) 6868
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78536
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).