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验证数据展示

  • WB analysis using 84480-2-RR (same clone as 84480-2-PBS)

    WB analysis using 84480-2-RR (same clone as 84480-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

    IF Staining of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

    FC experiment of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GSR Recombinant antibody (84480-2-RR, Clone:241934D2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01316-1

    Cytometric bead array standard curve of MP01316-1

    Cytometric bead array standard curve of MP01316-1, GSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84480-2-PBS. Detection antibody: 84480-1-PBS. Standard: Ag13080. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01316-2

    Cytometric bead array standard curve of MP01316-2

    Cytometric bead array standard curve of MP01316-2, GSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84480-2-PBS. Detection antibody: 84480-3-PBS. Standard: Ag13080. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84480-2-RR

    Affinity and Kinetic Characterization of 84480-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

GSR Recombinant antibody, PBS Only (Capture)

GSR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84480-2-PBS

产品说明书

CloneNo. 241934D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84480-2-PBS targets GSR as part of a matched antibody pair:

MP01316-1: 84480-2-PBS capture and 84480-1-PBS detection (validated in Cytometric bead array)

MP01316-2: 84480-2-PBS capture and 84480-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GSR fusion protein Ag13080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione reductase
别名 GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7
计算分子量 522 aa, 56 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC069244
基因名称 GSR
Gene ID (NCBI) 2936
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00390
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.