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验证数据展示

  • WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

    WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84892-1-RR (RFC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RFC2 Recombinant antibody (84892-1-RR, Clone:242503E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2, Human RFC2 Recombinant Matched Antibody Pair - PBS only. 84892-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0663. 84892-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01625-2

    Sample test of MP01625-2

    The mean RFC2 concentration was determined to be 55.11 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load, 130.72 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 43.49 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2, RFC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84892-1-PBS. Detection antibody: 84892-2-PBS. Standard: Ag0663. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84892-1-RR

    Affinity and Kinetic Characterization of 84892-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84892-1-RR against Human RFC2 were performed. The affinity constant is 0.775 nM.

RFC2 Recombinant antibody, PBS Only (Capture)

RFC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84892-1-PBS

产品说明书

CloneNo. 242503E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84892-1-PBS targets RFC2 as part of a matched antibody pair:

MP01625-2: 84892-1-PBS capture and 84892-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 RFC2 fusion protein Ag0663 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication factor C (activator 1) 2, 40kDa
别名 Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1
计算分子量 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC002813
基因名称 RFC2
Gene ID (NCBI) 5982
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35250
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).