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验证数据展示

  • FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01168-2

    Cytometric bead array standard curve of MP01168-2

    Cytometric bead array standard curve of MP01168-2, BTLA/CD272 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98096-1-PBS. Detection antibody: 84262-2-PBS. Standard: RP02326. Range: 0.078-10 ng/mL.

  • Affinity and Kinetic Characterization of 98096-1-RR

    Affinity and Kinetic Characterization of 98096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

Anti-Human BTLA/CD272 Rabbit Recombinant Antibody, PBS Only (Capture)

BTLA/CD272 Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA
Cat No. 98096-1-PBS

产品说明书

CloneNo. 241318H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Cytometric bead array, Indirect ELISA

别名

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98096-1-PBS targets BTLA/CD272 as part of a matched antibody pair:

MP01168-2: 98096-1-PBS capture and 84262-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator
计算分子量 289 aa, 33 kDa
GenBank蛋白编号BC107091
基因名称 BTLA
Gene ID (NCBI) 151888
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z6A9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.