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验证数据展示

  • FC experiment of C57BL/c mouse splenocytes using CL647-65139

    FC experiment of C57BL/c mouse splenocytes using CL647-65139

    1x10^6 C57BL/c mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45R (B220) (CL647-65139, Clone:RA3-6B2) or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were co-stained with PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65139

    IF Staining of mouse splenocytes using CL647-65139

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated CD45R (B220) antibody (CL647-65139, Clone: RA3-6B2, red) at dilution of 1:400, and CoraLite®488-conjugated CD3 antibody (CL488-65077, Clone: 17A2, green).

CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for IF/ICC, FC
Cat No. CL647-65139

产品说明书

CloneNo. RA3-6B2

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

IF/ICC, FC

别名

CD45R, Cd45, Ptprc, Leukocyte common antigen, LCA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65139-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65139 targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 Abelson murine leukemia virus-induced pre-B tumor cells 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 CD45R, Cd45, Ptprc, Leukocyte common antigen, LCA
GenBank蛋白编号BC028512
基因名称 CD45
Gene ID (NCBI) 19264
RRIDAB_2883705
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP06800
储存缓冲液 PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
FC protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols