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验证数据展示

  • WB analysis using 66464-1-Ig

    WB analysis using 66464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66464-1-Ig

    IF Staining of HeLa using 66464-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66464-1-Ig

产品说明书

CloneNo. 1A1A3

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Raji cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRM1 Ready-To-Use IHC Kit. ADRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66464-1-Ig targets ADRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ADRM1 fusion protein Ag24699 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 adhesion regulating molecule 1
别名 adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog
计算分子量 407 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC017245
基因名称 ADRM1
Gene ID (NCBI) 11047
RRIDAB_2881832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16186
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

实验方案

Product Specific Protocols
WB protocol for ADRM1 antibody 66464-1-IgDownload protocol
IF protocol for ADRM1 antibody 66464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols