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验证数据展示

  • WB analysis using 66464-1-Ig

    WB analysis using 66464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66464-1-Ig

    IF Staining of HeLa using 66464-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66464-1-Ig

Print datasheet

CloneNo. 1A1A3

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Raji cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRM1 Ready-To-Use IHC Kit. ADRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66464-1-Ig targets ADRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ADRM1 fusion protein Ag24699 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name adhesion regulating molecule 1
Synonyms adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog
Calculated Molecular Weight 407 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC017245
Gene Symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2881832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

实验方案

Product Specific Protocols
WB protocol for ADRM1 antibody 66464-1-IgDownload protocol
IF protocol for ADRM1 antibody 66464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols