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验证数据展示

  • WB analysis of human plasma using 66178-1-Ig

    WB analysis of human plasma using 66178-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66178-1-Ig (AZGP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66178-1-Ig

    IHC staining of human breast cancer using 66178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IHC staining of human breast cancer using 66178-1-Ig

    IHC staining of human breast cancer using 66178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IF Staining of human breast cancer using 66178-1-Ig

    IF Staining of human breast cancer using 66178-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Zinc Alpha 2 Glycoprotein antibody (66178-1-Ig, Clone: 4B8A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Zinc Alpha 2 Glycoprotein Monoclonal antibody

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66178-1-Ig

Print datasheet

CloneNo. 4B8A4
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AZGP1 Ready-To-Use IHC Kit. AZGP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Zinc Alpha 2 Glycoprotein fusion protein Ag5661 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-2-glycoprotein 1, zinc-binding
Synonyms AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC033830
Gene Symbol Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_2881573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

实验方案

Product Specific Protocols
WB protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
IF protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China.

Authors - Hong Tang
View Article