验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Zinc Alpha 2 Glycoprotein fusion protein Ag5661 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | alpha-2-glycoprotein 1, zinc-binding |
别名 | AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1 |
计算分子量 | 298 aa, 34 kDa |
观测分子量 | 41 kDa |
GenBank蛋白编号 | BC033830 |
基因名称 | Zinc Alpha 2 Glycoprotein |
Gene ID (NCBI) | 563 |
RRID | AB_2881573 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P25311 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
实验方案
Product Specific Protocols | |
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WB protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
IF protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |