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BRE Monoclonal antibody

BRE Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2E5A5

Cat no : 66356-1-Ig

Print datasheet

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

验证数据展示

  • WB analysis of fetal human brain using 66356-1-Ig

    WB analysis of fetal human brain using 66356-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 66356-1-Ig

    IF Staining of mouse brain using 66356-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRE antibody (66356-1-Ig, Clone: 2E5A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse brain using 66356-1-Ig

    IHC staining of mouse brain using 66356-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66356-1-Ig

    IHC staining of mouse brain using 66356-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66356-1-Ig targets BRE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen BRE fusion protein Ag2256 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name brain and reproductive organ-expressed (TNFRSF1A modulator)
Synonyms BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2
Calculated Molecular Weight 383 aa, 44 kDa
Observed Molecular Weight 42-47 kDa
GenBank Accession NumberBC001251
Gene Symbol BRE
Gene ID (NCBI) 9577
RRIDAB_2881735
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

实验方案

Product Specific Protocols
WB protocol for BRE antibody 66356-1-IgDownload protocol
IHC protocol for BRE antibody 66356-1-IgDownload protocol
IF protocol for BRE antibody 66356-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols