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验证数据展示

  • IF Staining of mouse brain using CL594-66356

    IF Staining of mouse brain using CL594-66356

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 BRE antibody (CL594-66356, Clone: 2E5A5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66356

    FC experiment of HeLa using CL594-66356

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated BRE Monoclonal antibody (CL594-66356, Clone:2E5A5)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated BRE Monoclonal antibody

BRE Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-66356

产品说明书

CloneNo. 2E5A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

BRCC4, BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 45, BRCA1 A complex subunit BRE, Brain and reproductive organ-expressed protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66356-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66356 targets BRE in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BRE fusion protein Ag2256 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 brain and reproductive organ-expressed (TNFRSF1A modulator)
别名 BRCC4, BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 45, BRCA1 A complex subunit BRE, Brain and reproductive organ-expressed protein
计算分子量 383 aa, 44 kDa
观测分子量 42-47 kDa
GenBank蛋白编号BC001251
基因名称 BRE
Gene ID (NCBI) 9577
RRIDAB_3084707
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXR7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 BRE antibody CL594-66356Download protocol
FC protocol for CL594 BRE antibody CL594-66356Download protocol
Standard Protocols
Click here to view our Standard Protocols