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Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody, PBS Only

CCL20/MIP-3 alpha Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241694B2

Cat no : 98221-1-PBS

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Synonyms

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

验证数据展示

  • FC experiment of THP-1 using 98221-1-RR (same clone as 98221-1-PBS)

    FC experiment of THP-1 using 98221-1-RR (same clone as 98221-1-PBS)

    1x10^6 PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98221-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CCL20/MIP3A Ready-To-Use IHC Kit. CCL20/MIP3A primary antibody included.

产品信息

98221-1-PBS targets CCL20/MIP-3 alpha in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 20
Synonyms CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20
Calculated Molecular Weight11kDa
GenBank Accession NumberNM_004591.3
Gene Symbol CCL20/MIP-3 alpha
Gene ID (NCBI) 6364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78556-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.