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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of THP-1 using 98221-1-RR

    FC experiment of THP-1 using 98221-1-RR

    1x10^6 PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated THP-1 cells were intracellularly stained with 0.25 ug Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody (98221-1-RR, Clone: 241694B2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Human CCL20/MIP-3 alpha Rabbit Recombinant Antibody

CCL20/MIP-3 alpha Recombinant Antibody for FC (Intra)
Cat No. 98221-1-RR
CloneNo. 241694B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPMA, LPS and Brefeldin A treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy CCL20/MIP3A Ready-To-Use IHC Kit. CCL20/MIP3A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98221-1-RR targets CCL20/MIP-3 alpha in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 20
Synonyms CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20
Calculated Molecular Weight11kDa
GenBank Accession NumberNM_004591.3
Gene Symbol CCL20/MIP-3 alpha
Gene ID (NCBI) 6364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78556-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

实验方案

Product Specific Protocols
FC protocol for CCL20/MIP-3 alpha antibody 98221-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols