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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of mouse splenocytes using FITC-65047

    FC experiment of mouse splenocytes using FITC-65047

    1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Anti-Mouse CD103 (2E7) (FITC-65047, Clone:2E7) or 0.25 ug Isotype Control. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

FITC Plus Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC
Cat No. FITC-65047

Print datasheet

CloneNo. 2E7

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Synonyms

Itgae, Integrin alpha M290, Integrin alpha E, aM290, alpha M290

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65047 targets CD103 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL) 种属同源性预测
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Full Name integrin alpha E, epithelial-associated
Synonyms Itgae, Integrin alpha M290, Integrin alpha E, aM290, alpha M290
GenBank Accession NumberBC150690
Gene Symbol CD103
Gene ID (NCBI) 16407
RRIDAB_2883739
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ60677
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD103 antibody FITC-65047Download protocol
Standard Protocols
Click here to view our Standard Protocols