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Biotin Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Biotin

CloneNo.

M1/70

Cat no : Biotin-65055

Print datasheet

Synonyms

Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

验证数据展示

  • FC experiment of mouse bone marrow cells using Biotin-65055

    FC experiment of mouse bone marrow cells using Biotin-65055

    1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.

  • FC experiment of mouse bone marrow cells using Biotin-65055

    FC experiment of mouse bone marrow cells using Biotin-65055

    1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse bone marrow cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65055 targets CD11b in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name integrin alpha M
Synonyms Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1
Calculated Molecular Weight127 kDa
GenBank Accession NumberBC156991
Gene Symbol CD11b
Gene ID (NCBI) 16409
Conjugate Biotin
Form Liquid
Purification MethodAffinity purification
UNIPROT IDE9Q604
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for Biotin CD11b antibody Biotin-65055Download protocol
Standard Protocols
Click here to view our Standard Protocols