• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of mouse splenocytes using 98024-1-RR

    FC experiment of mouse splenocytes using 98024-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone: 230371A7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98024-1-RR

    FC experiment of mouse splenocytes using 98024-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone:230371A7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98024-1-RR

    Affinity and Kinetic Characterization of 98024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98024-1 -RR against Mouse IL-6R alpha were performed. The affinity constant is 0.629 nM.

Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for FC
Cat No. 98024-1-RR
CloneNo. 230371A7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Il6ra, CD126, 230371A7, IL-6R alpha, Il6r

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98024-1-RR targets CD126/IL-6R alpha in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 receptor, alpha
Synonyms Il6ra, CD126, 230371A7, IL-6R alpha, Il6r
Calculated Molecular Weight50KD
GenBank Accession NumberNM_010559.3
Gene Symbol Il6ra
Gene ID (NCBI) 16194
RRIDAB_3672168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22272
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).

实验方案

Product Specific Protocols
FC protocol for CD126/IL-6R alpha antibody 98024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols