验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
推荐稀释比
Application | Dilution |
---|---|
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-98024 targets CD126/IL-6R alpha in FC applications and shows reactivity with mouse samples.
Tested Applications | FC Application Description |
Tested Reactivity | mouse |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | interleukin 6 receptor, alpha |
Synonyms | Il6ra, CD126, 230371A7, IL-6R alpha, Il6r |
Calculated Molecular Weight | 50KD |
GenBank Accession Number | NM_010559.3 |
Gene Symbol | Il6ra |
Gene ID (NCBI) | 16194 |
RRID | AB_3673369 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P22272 |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 CD126/IL-6R alpha antibody CL488-98024 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |